[ more parking ]
please note.... this is taken out of context of lots of relevant info.....
US5665543: Method of discovering chemicals capable of
functioning as gene expression modulators
Inventor(s):
Foulkes; J. Gordon , Huntington Station, NY
Leichtfried; Franz , Bellerose, NY
Pieler; Christian , Westbury, NY
Stephenson; John R. , Rockville Centre, NY
Franco; Robert , Spencerport, NY
Applicant(s):
Oncogene Science, Inc., Uniondale, NY
Issued/Filed Dates:
Sept. 9, 1997 / June 28, 1994
Application Number:
US1994000267834
IPC Class:
C12Q 001/68; C12N 015/85; C12P 021/02;
Class:
Current: 435/069.1; 435/320.1; 435/320.1;
Original: 435/006; 435/069.1; 435/320.1; 935/077; 935/078;
Field of Search:
435/6,91.2,172.3,69.1,320.1 935/77,78
Abstract:
The present invention provides a method of transcriptionally
modulating the expression of a gene-of-interest. The method
comprises contacting a cell which is capable of expressing the gene
with an amount of a molecule effective to transcriptionally modulate
expression of the gene and thereby affect the level of the protein
encoded by the gene which is expressed by the cell. Molecules
useful in the practice of the invention are characterized as follows (a)
do not naturally occur in the cell, (b) bind to DNA or RNA or bind to a
protein through a domain of such protein which is not a ligand binding
domain of a receptor which naturally occurs in the cell. Additionally,
this invention provides a method for determining whether a molecule
known to be a modulator of protein biosynthesis is capable of
transcriptionally modulating expression of a gene-of-interest.
First Claim:
What is claimed is:
1. A method of determining whether a chemical not previously known to be a modulator of protein
biosynthesis specifically transcriptionally modulates the expression of a gene-of-interest which
comprises:
(a) contacting a sample which contains a predefined number of identical eucaryotic cells with a
predetermined concentration of the chemical to be tested, each comprising a single DNA
construct consisting essentially of in 5' to 3' order
(i) a modulatable transcriptional regulatory sequence of the gene-of-interest,
(ii) a promoter of the gene-of-interest, and
(iii) a reporter gene which expresses a polypeptide that produces a detectable signal,
coupled to, and under the control of, the promoter, under conditions wherein the chemical
if capable of acting as a transcriptional modulator of the gene-of-interest, causes a
detectable signal to be produced by the polypeptide expressed by the reporter gene;
(b) quantitatively determining the amount of the signal produced in (a);
(c) comparing the amount of signal determined in (b) with the amount of signal produced and
detected in the absence of any chemical being tested and with the amount of signal produced
and detected upon contacting the sample in (a) with other chemicals, thereby identifying the test
chemical as a chemical which causes a change in the amount of detectable signal produced by
the polypeptide expressed by the reporter gene, and thereby determining whether the test
chemical specifically transcriptionally modulates expression of the gene-of-interest.
|
