Speaking of breakthroughs, last week this patent was issued:
United States Patent
5,723,329
Mangelsdorf, et. al.
Mar. 3, 1998
DNA encoding retinoid receptor X (RXR) alpha and gamma; corresponding vectors and host cells
Inventors:
Mangelsdorf; David J. (San Diego, CA); Evans; Ronald M. (La Jolla, CA).
Assignee:
The Salk Institute for Biological Studies (La Jolla, CA).
Appl. No.:
336,408
Filed:
Nov. 8, 1994
Related U.S. Application Data
Continuation of (including streamline cont.) Ser. No. 933,453, Aug. 21, 1992, abandoned, which is a continuation of
Ser. No. 478,071, Jan. 22, 1991, abandoned.
Intl. Cl. :
C12N 15/12, C12N 5/10
Current U.S. Cl.:
435/240.2; 435/326.1; 435/69.1; 435/69.7; 536/23.5
Field of Search:
536/23.5, 24.1; 435/69.1, 320.1, 240.2, 69.7
References Cited | [Referenced By]
U.S. Patent Documents
4,981,784
Jan., 1991
Evans et al.
435/6
5,171,671
Dec., 1992
Evans et al.
435/69.1
5,274,077
Dec., 1993
Evans et al.
530/350
5,403,925
Apr., 1995
Ozato
536/23.5
Other References
Mangelsdorf et al, Nature, v. 345, May 17, 1990, p. 224.
Hazel et al, Proc. Natl. Acad. Sci. USA, v. 85, Nov. 1988, p. 8444.
Giguere et al., "Identification of a receptor for the morphogen retinoic acid", Nature vol. 330:624-629 (1987).
Giguere et al., "Identification of a new class of steroid hormone receptors", Nature vol. 331:91-94 (1988).
Hazel et al., "A gene inducible by serum growth factors encodes a member steroid and thyroid hormone receptor
superfamily", Proc. natl. Acad. Sci. USA vol. 85:8444-8448 (1988).
Giguere et al., "Spatial and temporal expression of the retinoic acid receptor in the regenerating amphibian limb", Nature
vol. 337:566-569 (1989).
Hamada et al., "H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory
element of major histocompatibility class I genes and the estrogen response element", Proc. Natl. Acad. Sci. USA vol.
86:8289-8293 (1989).
Primary Examiner: Fitzgerald; David L.
Attorney, Agent or Firm: Pretty, Schroeder, Brueggemann & ClarkReiter; Stephen E.
Abstract
The present invention relates to novel receptor polypeptides, which, upon interaction with certain ligands, or activation by
certain compounds, modulate transcription of certain genes by binding to cognate response elements associated with promoters
of such genes. The novel receptors of the invention modulate transcription in the presence of retinoid compounds. The
receptors of the present invention differ significantly from known retinoic acid receptors, in protein primary sequence and in
responsiveness to exposure to various retinoids. The invention provides DNAs encoding the novel receptors, expression
vectors for expression of the receptors, cells transformed with such expression vectors, cells co-transformed with such
expression vectors and with reporter vectors to monitor modulation of transcription by the receptors, and methods of using
such co-transformed cells in screening for compounds which are capable, directly or indirectly, of activating the receptors. The
invention also provides nucleic acid probes for identifying DNAs which encode additional retinoid receptors of the same class
as the novel receptors disclosed herein.
17 Claims, 9 Drawing Figures |
