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Biotech / Medical : GZTC
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To: Rocketman who wrote (251)6/4/1997 9:50:00 PM
From: Robert Jensen   of 752
 
Refer back to post #225 I stated 200 to 400ul/pup-/My method:

Andy Carver states
100uL should be expected but 20uL would be acceptable.
Is that per teat or per mouse? Expensive machinery to get less?? Of course a vacuum pump will work but it still is an inefficient process. Mices are easy to raise. And really don't eat much. My way predicts 2 to 4X as much and on the high end predicts 20X as much leche. Maybe you don't need a lot... OK The problem of degradation via low ph is serious. Some proteins are less effected negatively. Some are little effected.
If your protein of interest does not fall into these categories this method is not for you.

Rocketman who was R. Ashman, is he at the Univ. of Iowa?

rjj
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