Here's the other abstract from Science:
Recovery of Replication-Competent HIV Despite
Prolonged Suppression of Plasma Viremia
Joseph K. Wong, * Marjan Hezareh, Huldrych F. Gnthard, Diane V. Havlir,
Caroline C. Ignacio, Celsa A. Spina, Douglas D. Richman
In evaluating current combination drug regimens for treatment of human immunodeficiency virus
(HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8
cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors,
activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the
isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to
fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new
drug resistance mutations or discernible evolution, providing evidence for viral latency rather than
drug failure.
J. K. Wong, M. Hezareh, H. F. Gnthard, C. C. Ignacio, C. A. Spina, Departments of Medicine
and Pathology, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La
Jolla, CA 92093, USA.
D. V. Havlir, Departments of Medicine and Pathology, University of California, San Diego, School
of Medicine, 9500 GIlman Drive, La Jolla, CA 92093, USA, and Treatment Center, University of
California, San Diego, 2760 Fifth Avenue, Suite 300, San Diego, CA 92103, USA.
D. D. Richman, Departments of Medicine and Pathology, University of California, San Diego,
School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA, and Veterans Affairs San
Diego Healthcare System, San Diego, CA 92063, USA.
* To whom correspondence should be addressed. |
