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Biotech / Medical : Gene therapy
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To: scaram(o)uche who wrote (157)9/13/1999 5:20:00 PM
From: RWReeves  Read Replies (2) of 319
 
For Mike;

You can use MAbs to harvest ("select") transfected cells or to purify target populations for your vector, but they don't have much of a role other than that. Still, it's enough that cell selection using MAbs is a big part of gene therapy and other "manipulation" (Great CBER term) strategies such as antigen pulsing of dendritics for vaccines.

CD34+ as Rick said is a marker on some types of "pluripotent progenitor cells" which can not only renew themselves but also differentiate into the whole panoply of blood cells, including platelets and all immune cells. It's believed CD34 is a homing marker which allows stem cells to find a parking place in the bone marrow.

CD34 surface marker doesn't do much in the way of activation. Seems to be just an anchor dragging for the right place to moor. There are other stem cell markers, including our beloved AC133, not yet assigned a CD determination, and one with as yet no name that is believed to be the holy grail of stem cells, a "CD34-" stem cell, which would be an even earlier progenitor of CD34 + cells.

Because CD34+ cells have this marvelous capability to renew and beget a whole range of other cell types, they make a pretty good target for gene therapy.

Getting pure CD34+ cells to mature and become specialized immune cells ex-vivo isn't all that easy and takes mucho growth factors and not a little black magic.

Hope that helps I can post a few links if you like.

Richard
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