Nat Biotechnol 1999 Sep;17(9):878-83
Genetic screens in yeast to identify mammalian nonreceptor modulators of
G-protein signaling.
Cismowski MJ, Takesono A, Ma C, Lizano JS, Xie X, Fuernkranz H, Lanier SM, Duzic E
Department of Pharmacology, Medical University of South Carolina, 171 Ashley Ave., Charleston, SC 29425, USA.
[Medline record in process]
We describe genetic screens in Saccharomyces cerevisiae designed to identify mammalian nonreceptor modulators of
G-protein signaling pathways. Strains lacking a pheromone-responsive G-protein coupled receptor and expressing a
mammalian-yeast Galpha hybrid protein were made conditional for growth upon either pheromone pathway activation
(activator screen) or pheromone pathway inactivation (inhibitor screen). Mammalian cDNAs that conferred plasmid-dependent
growth under restrictive conditions were identified. One of the cDNAs identified from the activator screen, a human
Ras-related G protein that we term AGS1 (for activator of G-protein signaling), appears to function by facilitating guanosine
triphosphate (GTP) exchange on the heterotrimeric Galpha. A cDNA product identified from the inhibitor screen encodes a
previously identified regulator of G-protein signaling, human RGS5. |
