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US5700657: Vectors and vector systems including
genes encoding tumor suppressor proteins and producer
cells transformed thereby
Inventor(s):
Beaudry; Gary A. , Montclair, NJ
Bertelsen; Arthur H. , Ridgewood, NJ
Sherman; Michael I. , GlenRidge, NJ
Vogelstein; Bert , Baltimore, MD
Applicant(s):
Genzyme Corporation, Framingham, MA
Issued/Filed Dates:
Dec. 23, 1997 / Dec. 13, 1993
Application Number:
US1993000166297
IPC Class:
C12P 021/02; C12P 019/34; C12N 005/10; C12N 007/01;
Class:
Current: 435/069.1; 435/091.33; 435/235.1; 435/325;
Original: 435/069.1; 435/091.33; 435/235.1; 435/325;
Field of Search:
435/69.1,69.2,172.1,172.3,320.1,240.2,235.1,325,91.33,91.3,91.31,91.32
Priority Number(s):
Dec. 13, 1993
US1993000166297
Abstract:
Vectors and vector systems are embodied that include a gene encoding a
tumor suppressor protein. In one embodiment, the vector includes a gene
encoding a tumor suppressor protein and an inducible promoter
controlling the gene encoding a tumor suppressor protein. The tumor
suppressor protein may be p53 protein. In another embodiment, there is
provided a vector system which comprises a first vector including a gene
encoding a tumor suppressor protein, and a second vector containing a
nucleic acid sequence encoding an antisense sequence complementary
to all or a portion of the gene encoding a tumor suppressor protein. In yet
another embodiment, there is provided a vector system which comprises
a first vector including a gene encoding a tumor suppressor protein, and a
second vector containing a gene which encodes a protein which binds to,
and thereby inhibits, the tumor suppressor protein. Such vectors and
vector systems enable cells transformed thereby to continue to proliferate
such that adequate amounts of infectious viral particles, generated from
viral vectors including a gene encoding a tumor suppressor protein, can
be harvested for administration to patients.
Attorney, Agent, or
Firm:
Olstein; E. M.; Lillie; R. J.;
Primary/Assistant
Examiners:
Guzo; David;
U.S. References:
(No patents reference this one)
Patent
Issued
Inventor(s)
Title
US4966847
10 /1990
McCormick et al.
Recombinant DNA clones containing a broad host range
gene from Bradyrhizobium japonicum
US5087617
2 /1992
Smith
Methods and compositions for treatment of cancer using
oligonucleotides
First Claim:
What is claimed is:
1. A process for producing a retroviral vector particle in a producer cell comprising:
producing a retroviral vector particle from a producer cell containing a retroviral vector including
RNA or DNA encoding a tumor suppressor protein wherein expression of said tumor suppressor
protein is controlled by an inducible promoter and said retroviral vector particle is produced under
conditions under which the inducible promoter is not activated. |
