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Biotech / Medical : Cadus Pharmaceutical Corp. (KDUS)

KDUS 1.600

0.0%

Jul 2 5:00 PM EST

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To: scaram(o)uche who wrote (23)	9/28/1999 8:51:00 PM
From: scaram(o)uche	Read Replies (1) of 1833

An abstract describing the efforts of Sklar (licensed by Axiom)..... Cytometry 1999 Oct 1;37(2):156-9 Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis. Edwards BS, Kuckuck F, Sklar LA Cancer Research and Treatment Center, Departments of Cytometry and Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico. [Medline record in process] Background:The tools for high throughput flow cytometry have been limited in part because of the requirement that the samples must flow under pressure. We describe a simple system for sampling repetitively from an open vessel. Methods:Under computer control, the sample is loaded into a sample loop in a reciprocating eight-way valve by the action of a syringe. When the valve position is switched, the plug of sample in the sample loop is transported to the flow cytometer by a pressure-driven fluid line. By coupling the plug-forming capability to a second multi-port valve, samples can be delivered sequentially from separate vessels. Results:The valve is able to deliver samples at rates ranging up to about 9 samples per minute. Each plug of sample has uniform delivery characteristics with a reproducible coefficient of variation (CV). Even at the highest sampling rate, carryover between samples is limited. Conclusions:Plug-flow flow cytometry has the potential to automate the delivery of small samples from unpressurized sources at rates compatible with many screening and assay applications. Copyright 1999 Wiley-Liss, Inc.

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