Don't remember having posted this before, please excuse duplication if I have. Priority date appears to be 4/26/94.....
Inventor(s):
Broach; James R. , Princeton, NJ
Manfredi; John P. , Ossining, NY
Trueheart; Joshua , Nyack, NY
Applicant(s):
Cadus Pharmaceutical Corporation, Tarrytown, NY
Issued/Filed Dates:
Dec. 14, 1999 / Jan. 14, 1997
Application Number:
US1997000732218
Abstract:
Mammalian adenylyl cyclases are functionally expressed in yeast
cells. The yeast cells may be used to screen for inhibitors or
activators of the adenylyl cyclase, or of a regulator of adenylyl
cyclase which is functionally co-expressed in the yeast cell. Methods
of identifying such inhibitors, activators and regulators are also
disclosed.
We claim:
1. A haploid yeast cell engineered to express a mammalian adenylyl cyclase and a mammalian or
chimeric G protein subunit, said mammalian adenylyl cyclase being functional and regulatable in said
cell even in the absence of expression of a mammalian G protein-coupled receptor by the yeast cell. |
