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Biotech / Medical : Cambridge Antibody Technology Group

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To: michael_f_murphy who wrote (7)	5/30/2000 7:35:00 AM
From: nigel bates	Read Replies (1) of 625

From healthtech.com (thanks Jongmans) " ... Protein Chips and Phage Display Dr. Scott Chappel, Senior Vice President of Research, Dyax Corporation Gene chip technology has allowed scientists to measure rates of the transcription of a collection of a large number of genes at once after a specific stimulus. While valuable, the accumulation of this information does not provide a complete picture of cellular responses to stimuli. This is due to the fact that the measurement of rates of gene transcription, may not always reflect the cell's response to a perturbation. Many stimuli are known to activate or deactivate existing proteins (for instance by phosphorylation or dephosphorylation) with no measurable change in expression rate of these proteins. An ideal method to study the responses of a cell to a stimulus would provide the ability to identify changes in the absolute amounts of specific proteins, as well as detect changes in the co- and post-translational modifications of those proteins. This can be accomplished by the identification of binding proteins to all intracellular proteins, and attaching them in a microarray format in a fashion analogous to gene chips. Such binding proteins with a high degree of specificity can be obtained through the use of phage display technology. Using this technology, antibodies can be made (completely in vitro) to any target. Moreover, selection methods allow for the identification of phage antibodies that recognize specific co- and/or post-translational modifications of the same protein. There are obvious synergies between the use of microarray chip technology and phage display isolation of specific binders to specific proteins. These technologies can be exploited to build protein chips that can quantitate changes in hundreds of proteins at once following any perturbation. Our efforts in this area will be discussed."

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