From the website -
"Phylos’ proprietary PROfusion™ technology enables the selection of peptides and proteins with desired properties. The fundamental advance represented by this unique platform is the in vitro covalent linkage of a protein (phenotype) to its encoding messenger RNA (genotype). This linkage permits selection of a protein based on its characteristics, as well as amplification of that protein through PCR, an efficient means of bringing the desired proteins to easily detectable levels.
The Advantages of PROfusion™
A powerful, rapid, and versatile solution to the selection of peptides and proteins from both natural and synthetic libraries, the PROfusion™ technology offers a number of superior characteristics:
* Library size
* Library complexity
* Speed
* Flexibility
* Versatility
* High-throughput
Creating Fusions: the PROfusion™ Molecule
The unique fusion molecule, which consists of a protein covalently linked to its own messenger RNA, is the basic unit of the PROfusion™ system. Starting with synthetic, semi-synthetic, and/or cellular DNA, Phylos has created libraries of up to 100 trillion proteins using the PROfusion™ technology. These libraries will provide capture proteins for PROfile™ chips and launch a new era of drug discovery and development. ...
Figure 1: Synthesis of the PROfusion™ Molecule
(A) A Puromycin is covalently attached to the 3' end of mRNA using a synthetic linker. During in vitro translation, a ribosome moves along the mRNA to generate a nascent protein.
(B) The C-terminus of the nascent protein covalently links to the puromycin, and the fusion is purified from the lysate containing the ribosomes. The protein is thus bound to its own mRNA, resulting in the PROfusion molecule.
Phylos has developed a powerful selection strategy to rapidly and efficiently select protein structures with the desired characteristics....
PROfusion™ Applications
Phylos has developed a number of PROfusion™ applications for internal programs as well as for collaborations with partners:
* An automated, high-throughput selection platform that can generate genome-scale high-affinity peptides/proteins, for the PROfile™ chip as well as for other applications in drug discovery
* Cellular protein libraries, for the elucidation of drug:protein, protein:protein, and enzyme:substrate interactions
* Protein libraries, for the development of human therapeutics
* Linear and constrained loop peptide libraries, for ligand generation, enzyme:substrate interaction, peptidomimetic design, and epitope mapping ..." |
