Publc/private cDNA clone library -
genomeweb.com
They are presenting at an interesting looking conference in SJ in June (anyone attending ?) -
beyondgenome.com
The next great challenge following the completion of the genome projects is to define the functions of all proteins. As the first step toward this goal, the Institute of Proteomics at Harvard Medical School is building a large-scale, state-of-the-art repository of all known genes and predicted open reading frames from human and other model organisms. The FLEX™ (Full-Length EXpression) Repository is prepared in a format that enables the transfer of hundreds or thousands of genes into any (or many) expression vector(s) in a single overnight step. The FLEX™ Repository will obviate a major roadblock in high-throughput protein analysis by facilitating high-throughput protein expression studies. In the modern paradigm, protein function experiments begin with the expression of the target protein in one of a variety of experimental contexts. This approach requires available copies of the studied cDNAs that are subcloned in-frame in appropriate expression vectors. Using traditional methods, this step is tedious when performed on a single gene and would not be feasible for hundreds of genes. With the genes in FLEX™, this could be accomplished for hundreds of genes in an overnight step. Moreover, the cDNAs are configured to allow the preparation of proteins with or without N- and/or C-terminal fusion peptides as needed. The clones in the FLEX™ Repository are acquired using in-house software to predict PCR primers from available sequence resources. The genes are then amplified by PCR from normal tissue cDNA libraries using automated protocols and then captured by recombinational cloning. The Institute has now completed its automation development phases and has a fully functional tracking database and workstation automation with the capacity to process >400 clones per week before sequencing. At present, there are over 1,500 clones in the human repository, and a first pass of the budding yeast genome has been made with 93% efficiency.
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List of those presenting -
SESSION CHAIRS
Dr. Joshua LaBaer, Harvard Medical School
Dr. Nathan Goodman, 3rd Millennium, Inc.
Dr. John Yates, The Scripps Research Institute
ADDITIONAL SPEAKERS
Dr. N. Leigh Anderson, Large Scale Proteomics Corporation
Dr. John J.M. Bergeron, McGill University
and Caprion Pharmaceuticals, Inc.
Dr. Remy Cromer, SurroMed, Inc.
Dr. James I. Garrels, Incyte Genomics, Inc.
Dr. Brad Guild, Millennium Pharmaceuticals, Inc.
Dr. Karen Heichman, Myriad Genetics, Inc.
Dr. Stephan Heymann, Kelman GmbH
Dr. Gordon Holt, Oxford GlycoSciences
Dr. Stephen Kingsmore, Molecular Staging Inc.
Dr. Brent L. Kreider, Phylos, Inc.
Dr. Joshua LaBaer, Harvard Medical School
Dr. Oded S. Lieberman, Proteologics, Inc.
Dr. Matthias Mann, MDS Proteomics
Dr. Michael Milburn, Structural GenomiX, Inc.
Dr. Jocelyn H. Ng, Xerion Pharmaceuticals GmbH
Dr. Mark Pearson, MDS Proteomics, Inc.
Dr. Simon Pitchford, Signature BioScience, Inc.
Dr. William E. Rich, Ciphergen Biosystems, Inc.
Dr. John D. Rodwell, AxCell Biosciences
Dr. Donny Strosberg, Hybrigenics
Dr. Cathy H. Wu, Georgetown University Medical Center
Dr. Michael G. Zwick, Promega Corporation |
