Wilder,
That looks recent and PubMed didn't find it. When and where is it from? Some upcoming conference? PubMed did find this; also new, but not as exciting or edifying.
>>J Microbiol Methods 2001 Aug;46(2):87-97 Related Articles, Books, LinkOut
Use of surface-enhanced laser desorption ionization protein chip system to analyze streptococcal exotoxin B activity secreted by Streptococcus pyogenes.
Boyle MD, Romer TG, Meeker AK, Sledjeski DD.
Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Avenue, 43614-5806, Toledo, OH, USA
Ciphergen surface-enhanced laser desorption ionization (SELDI) protein chip technology was used to analyze the secretion and autoactivation of the Streptococcus pyogenes cysteine protease SpeB. This method allowed rapid identification of both the zymogen form of the protein Mr approximately 41,000 and the fully active enzyme Mr approximately 28,500. SpeB production in culture supernatants was demonstrated to be growth-phase regulated and SpeB positive and negative variants of a blood passaged S. pyogenes isolate could readily be distinguished. In kinetic studies of the autoactivation of the zymogen form of SpeB, the sequential generation of four intermediates was detected before the accumulation of the fully active enzyme. The methods described enabled enhanced speed, use of lower sample volumes and concentrations, and a more complete molecular characterization of SpeB than allowed by existing methods of analysis using SDS-PAGE and Western immunoblotting.
Cheers, Tuck |
