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Biotech / Medical : PROTEOMICS

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To: keokalani'nui who wrote (344)	9/25/2001 12:54:31 AM
From: scaram(o)uche	of 539

Thanks, that was great. Robert: Let's switch gears and talk about pre-processing. Are there ways that you can pre-process and separate out collections of peptides or proteins to enhance the signal? Dr. Aebersold: Yes.You cannot take a proteome comprising several thousand proteins and simply infuse the mixture into a mass spectrometer and hope that the mixture would give you any useful information. You have to do pre-processing. Again, there are basically two tracks. It’s either pre-processing on the protein level or pre-processing on the peptide level, or some combination of both.............. A common implementation of peptide separation upstream of a mass spectrometer is ion exchange chromatography followed by reverse-phase chromatography. Jim Silverman and I were standing in front of the MLNM mass specs three years ago, getting a description of the HPLC interface.

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