I hadn't seen any indication, for years, that there was an in-house effort with HCV. This might just be some sort of hobby research, or could, I guess, be somehow unrelated to an interest in HCV. Unlikely, IMO.......
Virus Genes 2001;23(2):149-55
Characterization of RNA synthesis and translation of bovine viral diarrhea virus (BVDV).
Li Y, McNally J.
Tularik Inc., South San Francisco, CA 94080, USA. yli@tularik.com
Full length and replicon genomes of various strains of bovine viral diarrhea virus (BVDV) have been characterized. Analysis of growth kinetics for a pair of cytopathogenic (cp) and noncytopathogenic (ncp) strains revealed that ncp strain synthesized viral RNA at much reduced level compared to the cp strain. Kinetics of translation and replication, the effects of bi-cistronic versus mono-cistronic genomes, and cis requirements for viral replication were also examined in a BVDV replicon D19c. Importantly, our results suggest a tight regulation and a switch from translation to replication, and demonstrated the cis requirements of NS4B and NS5A in replication. |
