from the IBC international conference, phila Oct 27-31
The World's Premier Microtechnology Event for Life Sciences
chipstohits.com
00 Knowledge-Based Template Optimization for High-Throughput Protein Expression
Dr. Wolfgang Mutter, Director, Research & Development, Roche Applied Science, Germany
The demand for a fast and parallel protein expression system has become greater than ever. However, traditional recombinant DNA technology is not likely to keep pace with the exploding amount of genetic information. As an alternative technology we established a cell free high-throughput expression system which uses a PCR generated template for protein expression. With this system we can express hundreds of proteins in parallel. In order to improve the reliability and success rate of protein expression through the rational design of the template, we have developed a knowledge-based software called ProteoExpert. This software calculates the secondary structure of the mRNA and provides higher success rate and higher protein yield.
4:30 Data on the Whole Sample Preparation Process for One and Two-Dimensional Gels, Liquid Chromatography, and Mass Spectrometry
Dr. Hanno Langen, F. Hoffmann-La Roche Ag, Germany
Sample preparation is one of the most important aspects for proteomics. The first step is the correct sample selection. Depending on the technology used the sample has to be prepared and contaminants which might be detrimental should be removed. I will show data from our proteomics group about the whole process from sample preparation for one and two dimensional gels, liquid chromatography, and finally mass spectrometry. Especially when the sample is transferred from one step to the other special precautions have to be taken so that the sample is suitable for the next step.
and just to make sure they understand it Roche will have the Combi presentation repeated 6 times over the 4 day show.
:00 Refreshment Break and Product Demonstrations [] CombiMatrix' Fully Integrated Microarray Platform: A Flexible and Rapid System for Customized Oligonucleotide Arrays
Dr. Andy McShea, Director of Applied Science, CombiMatrix Corporation
The CombiMatrix System, available from Roche Applied Science, integrates software for designing custom microarrays, a semiconductor-based chip on which oligonucleotides are synthesized in situ, a hybridizer-reader instrument, benchtop microarray synthesizer, and software for data acquisition, recording, and analysis. Microarrays can be produced within 48 hours, and users can customize the content on every chip. |
