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Biotech / Medical : Oxford GlycoSciences Plc
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To: scaram(o)uche who wrote (292)12/28/2002 5:52:44 PM
From: scaram(o)uche   of 469
 
Foreign Application Priority Data......... Nov 29, 1996[GB]

patft.uspto.gov;

What is claimed is:

1. A computer-assisted method for selecting and directing the isolation of one or more proteins present in a two-dimensional array, comprising:

a purification step, wherein a biological sample is treated by selectively depleting the sample of one or more proteins;

a first separation step following the purification step, wherein a plurality of proteins in the treated sample are separated according to a first physical or chemical property to form a one-dimensional array of proteins;

a second separation step, wherein the one-dimensional array of proteins is separated according to a second physical or chemical property to form the two-dimensional array;

imaging the two-dimensional array or a replica thereof to generate a computer-readable output comprising, for each of a plurality of proteins detected in the two-dimensional array, a pair of x,y coordinates and a signal value;

processing the output in at least one computer to select one or more of the detected proteins in accordance with previously ordained or operator-specified criteria; and

generating machine-readable instructions that direct a robotic device to isolate at least one of the selected proteins from the two-dimensional array.
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