United States Patent Application 20030008380
Kind Code A1
FOWLKES, DANA MERRIMAN ; et al. January 9, 2003
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Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
Abstract
Yeast cells are engineered to express both a surrogate of a pheromone system protein (e.g., enzymes involved in maturation of .alpha.-factor, transporters of a-factor, pheromone receptors, etc.) and a potential peptide modulator of the surrogate, in such a manner that the inhibition or activation of the surrogate affects a screenable or selectable trait of the yeast cells. Various additional features improve the signal-to-noise ratio of the screening/selection system.
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Inventors: FOWLKES, DANA MERRIMAN; (CHAPEL HILL, NC) ; BROACH, JIM; (PRINCETON, NJ) ; MANFREDI, JOHN; (NEW YORK, NY) ; KLEIN, CHRISTINE; (NEW YORK, NY) ; MURPHY, ANDREW J.; (MONTCLAIR, NJ) ; PAUL, DR. JEREMY; (SOUTH NYACK, NY) ; TRUEHEART, JOSHUA; (SOUTH NYACK, NY)
Correspondence Name and Address: GIULIO A. DECONTI, JR.
LAHIVE & COCKFIELD, LLP
28 STATE STREET
BOSTON
MA
02109
US
Serial No.: 309196
Series Code: 09
Filed: May 10, 1999
(for this one, Cadus is not specified as the assignee. however, it was filed on the same day as US2003009022 by the same attorneys.) |
