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Biotech / Medical : The thread of life
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To: tuck who wrote (476)5/31/2003 11:40:31 AM
From: Mike McFarlandRead Replies (2) of 1336
 
I read here
med.unc.edu
that there are lentivirus proteins toxic to
the cell lines, as well as the toxic VSV used
to pseudotype. Sure enough here is part of what
they do (from Baltimore patent). Of course, 98%
of this is going over my head, but thanks for
the patents!

[0097] In the preferred embodiment a packaging cell line that does not stably express viral proteins is transfected with the viral construct, a second vector comprising the HIV-1 packaging vector with the env, nef, 5'LTR, 3'LTR and vpu sequences deleted, and a third vector encoding an envelope glycoprotein. Preferably the third vector encodes the VSVg envelope glycoprotein.

Hell, I probably wont even get to my Rigel stuff
which finally showed up in the mail yesterday.
Thanks again Tuck!

Oh, interesting tidbit: Garry Nolan (who was one of
of the founders of Rigel) does this stuff.
You can visit his lab here:
stanford.edu
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