>>LEXG must really hate this stuff... <<
Interesting point. I wonder if they'd try to license something like this. Here's the abstract, which doesn't add much to the article you posted except that dodging the interferon response is done by essentially chopping off the tails.
>>GENES & DEVELOPMENT 17:1340-1345, 2003
ISSN 0890-9369/
RESEARCH COMMUNICATION
Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter
Toshie Shinagawa and Shunsuke Ishii1
Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan
We have developed a new vector, named pDECAP, to express long double-strand RNA (ds-RNA) from an RNA polymerase II (Pol II) promoter. Because the transcripts from pDECAP lack both the 5'-cap structure and the 3'-poly(A) tail that facilitate ds-RNA export to the cytoplasm, long ds-RNA from pDECAP does not induce the interferon response. Transgenic mice embryos expressing long ds-RNA for the transcriptional corepressor Ski from this vector exhibited phenotypes that were remarkably similar to those of Ski-deficient embryos, including defects of neural tube closure and eye formation. Thus, this vector provides a new tool to efficiently generate tissue-specific knockdown mice for studying gene function in whole animal systems. <<
Cheers, Tuck |
