>>The Sequence of SARS Mutations
« on: Today at 07:59:33am »
niman
The updated sequences of GZ01 (including the sequence of the 122 aa protein created by the 29 nt insert), BJ02, BJ03, BJ04 were just made available at GeneBank.
The completed genomes of BJ02, BJ03, BJ04, GZ01 allow for a full data set for the 5 Metropole Hotel Mutations. Not surprisingly, none are found in GZ01, BJ02, BJ03. However, 2 are found in BJ04 (C9404T and A21721G).
BJ04 is the first reported isolate that has only some of the 5 Metropole Hotel Mutations. All 5 mutations are found in Tor2, Urbani, HKU39849, SIN2500, SIN2677, SIN2679, SIN2740, SIN2774, CUHK-Su10, TW1, ZJ01. None of the 5 mutations are found in GZ01, BJ01, BJ02, BJ03, CUHK-W1.
Details on the BJ04 patient are not widely known, but since the BJ04 isolate was the most recent submission to GeneBank by the Beijing Genomics Institute and at the time of initial submission it was the least completed sequence, it seems possible that the isolate was from a patient who developed SARS later than patients from whom GZ01, BJ01, BJ02, BJ03 were isolated by the Beijing Genomics Institute.
Therefore, it seems that evolution of the viruses isolated from visitors to the Metropole Hotel may have gone through the following sequence of mutations.
29 nt deletion - found in all isolates except GZ01
Two Metropole Hotel Mutations (C9404T, A21721G) - found in BJ04
Three additional Metropole Mutations (G17564T, C22222T, C27827T) - all 5 Metropole Hotel mutations found in the 11 isolates listed above
Posts: 21
Re:The Sequence of SARS Mutations
« Reply #1 on: Today at 12:44:15pm »
niman
I have had a chance to look at the revised sequence some more and now it looks like there are 7 Metropole Hotel mutations, in addition to the 29 nt delection. Two of the mutation which were initially just found in Beijing isolates have now also been found in GZ01. Thus, it seems that the sequence of mutations is as follows:
29 nt deletion (found in all isolates except GZ01)
Two mutations (A19838G and C27243T) in CUHK-W1
Two mutations (C9404T and A21721G) in BJ04
Combining the 4 mutations above with G17565T, C22222T, and C27827T to produce the 11 isolates with all 7 mutations plus the 29 nt deletion.<<
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