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Pastimes : SARS - what next?
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To: Ilaine who wrote (543)6/17/2003 1:51:09 PM
From: Henry Niman  Read Replies (1) of 1070
 
Yes, PCR amplifies the target, so additional amplifications can turn a weak positive into a strong positive. The most common source of false positives in a PCR test is carry over of material from a positive sample to a negative sample. the cross contaminating material is amplified to give a false positive.

However, this potential problem is well known to labs that routinely run PCR tests, and protocols are designed to minimize or eliminate such carry over.
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