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Biotech / Medical : Guilford (GLFD) - Steadily Rising
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To: scaram(o)uche who wrote (442)9/11/2003 8:58:43 AM
From: scaram(o)uche  Read Replies (3) of 496
 
J Biol Chem. 2003 Sep 5 [Epub ahead of print].

Transcriptional coactivation of NF-kappa B-dependent gene expression by p300 is regulated by PARP-1.

Hassa PO, Buerki C, Lombardi C, Imhof R, Hottiger MO.

Institute of Veterinary Biochemistry and Molecular Biology, Zurich 8057.

Nuclear factor kappaB (NF-kappaB) plays an important role in the transcriptional regulation of genes involved in inflammation and cell survival. Here, we demonstrate that NF-kappaB-dependent gene expression was inhibited by E1A in poly(ADP)-ribose polymerase-1 knock out (PARP-1 (-/-)) cells complemented with wild type PARP-1 after TNFalpha or LPS treatment. PARP-1 and p300 synergistically coactivated NF-kappaB-dependent gene expression in response to TNFalpha and LPS. Furthermore, PARP-1 interacted directly with p300 and enhanced the interaction of NF-kappaB1/p50 to p300. The C-terminus, harboring the catalytic domain of PARP-1 but not its enzymatic activity was required for complete transcriptional coactivation of NF-kappaB by p300 in response to TNFalpha and LPS. Together, these results indicate that PARP-1 acts synergistically with p300 and plays an essential regulatory role in NF-kappaB-dependent gene expression.

(no direct relevance, that I know of, to anything ongoing at GLFD. Just parking.)
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