Discovering the secrets of Hsp90 binding
A ubiquitous antitumor drug target preferentially kills tumor cells due to target conformation | By David Secko
Geldanamycin is an antibiotic that binds to Hsp90 and inhibits its adenosine triphosphate binding and activity as a chaperone. A derivative of geldanamycin (GM) is the Hsp90 inhibitor 17-allylaminogeldanamycin (17-AAG), which preferentially kills tumor cells and is in phase I clinical trials. Hsp90 is found in all cell types, but it has been unclear how the preferential killing of tumor cells by 17-AAG is accomplished. In the September 25 Nature, Adeela Kamal and colleagues at Conforma Therapeutics Corporation report that Hsp90 from tumor cells binds with higher affinity to 17-AAG and that this is due to Hsp90 being in multichaperone complexes in these cells. (Nature, 425:407-410, September 25, 2003)
Kamal et al. performed competitive binding assays using increasing concentrations of 17-AAG to inhibit the binding of Hsp90 to GM-biotin and observed that Hsp90 from tumor cells had a 50% inhibitory concentration (IC50) of 6 nM compared with 400?600 nM for Hsp90 from control cells. Measurements comparing the binding of adenosine triphosphate (ATP) to Hsp90 also showed a lower IC50 for tumor Hsp90, revealing that Hsp90 in tumor cells had a significantly higher binding affinity for 17-AAG. Hsp90 assembles in multichaperone complexes by interacting with many cochaperone proteins (e.g., p23, Hop), and co-immunoprecipitation of Hsp90 demonstrated that Hsp90 from tumor cells is present in more of these complexes. Hsp90 tumor complexes also showed a higher ATPase activity (something that Hsp90 chaperone function is dependent on) that was inhibited by 17-AAG. In addition, in vitro reconstituted Hsp90 complexes showed an increased apparent affinity for 17-AAG?12 nM for Hsp90 alone rising to 600 nM when complexed. The authors then performed these experiments in mice and in clinical cancer and observed a similar pattern of 17-AAG binding to in vivo tumor Hsp90.
?Our data show that Hsp90 in tumor cells exists in a functionally distinct molecular form. [Hsp90 could be the] Achilles heel of tumor cells, driving the selective accumulation and bioactivity of pharmacological Hsp90 inhibitors, and making tumor Hsp90 a unique cancer target,? conclude the authors.
Links for this article
L. Neckers, Y.S. Lee, ?The rules of attraction,? Nature, 425:357-358, September 25, 2003.
nature.com
J.S. Isaacs et al., ?Heat shock protein 90 as a molecular target for cancer therapeutics,? Cancer Cell, 3:213-217, March 2003.
[PubMed Abstract]
A. Kamal et al., ?A high-affinity conformation of Hsp90 confers tumor selectivity on Hsp90 inhibitors,? Nature, 425:407-410, September 25, 2003.
nature.com
Conforma Therapeutics
conformacorp.com
K. Richter, J. Buchner, ?Hsp90: chaperoning signal transduction,? Journal of Cell Physiology, 188:281-290, September 2003.
[PubMed Abstract]
biomedcentral.com |
