HOT>>XTRN patent #6,605,451 I was just trolling for some data, and I find that this patent was issued on 12 August, 2003, and as usual, they didn't say anything.
Not a peep.
Now why would you keep this a secret & not put this news out???
They have some new product applications out too, and not a peep...
As I thought, someone has been sucking up the cheap shares, so this sure has the markings of a skunk/sandbag.
This is a pretty big deal Imo, it means more barrier of entry for others, just like I said with BRLI & BDY years back, when they were thought to be all washed up...
Anyway, here it is>
patft.uspto.gov
And here is a short summary>
The present invention pertains to methods, reagents, compositions, kits, and instruments for use in simultaneously amplifying multiple targets. In particular, this invention is based on the discovery of a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to "boost" the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid.
SUMMARY OF THE INVENTION
Accordingly, it is a general object of this invention to provide a method that permits the multiplex amplification of multiple targets without extensive design and optimization constraints.
Another object of the invention is to provide a method that enables amplification of multiple targets (or multiple amplification assays) from limited quantity specimens with very low nucleic acid copy number.
A further object of the invention is to provide a diagnostic kit that allows the user to perform amplification of multiple targets without extensive design and optimization constraints and to amplify targets from limited quantity specimens with very low nucleic acid copy number.
Additional objects, advantages and novel features of this invention shall be set forth in part in the description that follows, and in part will become apparent to those skilled in the art upon examination of the following specification or may be learned by the practice of the invention. The objects and advantages of the invention may be realized and attained by means of the instrumentalities, combinations, compositions, and methods particularly pointed out in the appended claims.
To achieve the foregoing and other objects and in accordance with the purposes of the present invention, as embodied and broadly described therein, the method of this invention comprises a two-step multiplex amplification reaction wherein the first step truncates a standard multiplex amplification round thereby resulting in a product having a boosted target copy number while minimizing primer artifacts. The second step divides the resulting product achieved in the first step into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first step.
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Field/mobile usable bio-warfare & food pathogen detection comes to mind..
To date, the Company has received $4.5 million in federal grants and contracts and $1.2 million in private investments to further commercialize specific food, water, environmental, clinical, and defense applications for the Company’s nucleic acid testing technologies. In the area of nucleic acid detection, we currently have 4 issued patents, and seven additional patents have been filed |
