>>J Nat Prod. 2005 Apr;68(4):607-10.
Production of 8-Demethylgeldanamycin and 4,5-Epoxy-8-demethylgeldanamycin from a Recombinant Strain of Streptomyces hygroscopicus.
Buchanan GO, Regentin R, Piagentini M, Rascher A, McDaniel R, Galazzo JL, Licari PJ.
Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545.
Two new geldanamycin derivatives produced by genetic engineering of Streptomyces hygroscopicus strain K309-27-1 were isolated and characterized. Removal of the 8-methyl group of geldanamycin was achieved by replacing the AT4 domain of the polyketide synthase with a malonyl AT domain. The resulting strain produced 8-demethyl geldanamycin (2) and 4,5-epoxy-8-demethylgeldanamycin (3). The structures of both molecules were elucidated through interpretation of 1D and 2D NMR data as well as comparison with authentic geldanamycin derivatives. Compounds 2 and 3 displayed moderate cytotoxicity against the human breast cancer cell line SK-BR-3.<<
Probably not going to amount to anything, but parking, anyway.
Cheers, Tuck |
