Misc stuff about their Kans:
IRORI’s NanoKan system extends encoding
to the use of machine readable barcodes and theoretically
allows libraries of up to 10,000 members to be prepared
bentham-science.org
Temperature/Stability profile of IRORI Kans by
Pui Yee Ng
see page 20
biotagedcg.com
"However, from the perspective of our research and training
goals in chemical genetics, the IRORI system suffers from two
shortcomings, both relating to cost. First, given our ability
to perform highly miniaturized and effective phenotypic [5]
and protein-binding assays [6,7], we calculate that an optimal
sample size of compound derived from an individual bead (of
which a Kan is a functional equivalent) is closer to 0.1 mg.
Since the majority of compounds synthesized in a split-pool
synthesis will not score as a positive in an assay, over-
synthesis is costly and wasteful. Second, we aimed to develop
a simple and inexpensive system, one, for example, that could
be re-established by a trainee at a second site within the
constraints of a typical academic budget. If chemical
genetics is to have the broad applicability of biochemistry
and genetics as a vehicle to explore biology, it must be
portable, accessible, and costcontained. This is an issue
that also faces genomics as it evolves into a broadly
applicable means to explore biology."
broad.mit.edu |
