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Biotech / Medical : momo-T/FIF

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To: tuck who wrote (2934)	8/12/2005 12:39:03 PM
From: former_pgs	Read Replies (1) of 12215

OT: I realize that you wanted an ELISA, but... It seems that focusing the protein by 2D SDS-PAGE or resolving it by 1D SDS-PAGE (you can fiddle with the gel concentrations to resolve) followed by Western blotting with an antibody against a common part of the protein (that is not cleaved off) would be more powerful than the ELISA. The detection limit / sensitivity would be basically the same, but the above two methods would be more powerful in that you would get some absolute numbers regarding the % of the protein cleaved. ELISA won't give you such numbers unless you know the affinity of the antibodies that you're using. Just in case you are not married to the ELISA technique... the above still qualifies as an immunoassay of sorts ;-)

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