[Biomarker discovery for ovarian cancer using SELDI-TOF-MS]
>>Gynecol Oncol. 2006 Jan 3; [Epub ahead of print]
Biomarker discovery for ovarian cancer using SELDI-TOF-MS.
Zhang H, Kong B, Qu X, Jia L, Deng B, Yang Q.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Ji'nan 250012, Shandong Province, P.R. China.
OBJECTIVES.: The purpose of this study is to discover potential biomarkers for the detection and monitoring of adjuvant chemotherapy for ovarian cancer. METHODS.: Serum samples from ovarian cancers and non-cancer controls were analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). To discover the possible diagnostic biomarker for ovarian cancer, a preliminary training set of spectra derived from 31 primary ovarian cancer patients, 16 patients with benign ovarian diseases, and 25 healthy women was used to develop a proteomic model that discriminated cancer from non-cancer effectively. A blind test set, including 43 new cases, was used to validate the sensitivity and specificity of this multivariate model. To explore treatment-induced serum protein change, the protein profiles generated from 16 postoperative patients before chemotherapy are compared with those obtained after chemotherapy. RESULTS.: A Four-peak model was established in the training set that discriminated cancer from non-cancer with sensitivity of 90.8% and specificity of 93.5%. A sensitivity of 87.0% and a specificity of 95.0% for the blind test were obtained, compared with 60.7%, 55% for CA125 for the same samples. These 4 markers performed significantly better than the current standard marker, CA125 (P < 0.05). One protein peak (mass/charge ratio [m/z], 4475) was identified in 12 of 16 (75%) postoperative patients after chemotherapy, but was absent before chemotherapy. CONCLUSION.: The proteins represented by these peaks are candidate biomarkers for ovarian cancer diagnosis and/or monitoring treatment response.<<
Cheers, Tuck |
