[SELDI-TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions]
>>Proteomics. 2006 Jan 30; [Epub ahead of print]
SELDI-TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions.
Traum AZ, Wells MP, Aivado M, Libermann TA, Ramoni MF, Schachter AD.
Division of Nephrology, Children's Hospital Boston, Boston, MA, USA.
Proteomic profiling with SELDI-TOF MS has facilitated the discovery of disease-specific protein profiles. However, multicenter studies are often hindered by the logistics required for prompt deep-freezing of samples in liquid nitrogen or dry ice within the clinic setting prior to shipping. We report high concordance between MS profiles within sets of quadruplicate split urine and serum samples deep-frozen at 0, 2, 6, and 24 h after sample collection. Gage R&R results confirm that deep-freezing times are not a statistically significant source of SELDI-TOF MS variability for either blood or urine.<<
Hi, Spec,
Ms. Page once told me snap-freezing wasn't needed, and this would seem to back her up. And the SARS news? Found that over a week ago . . .
Cheers, Tuck |
