Dr. SERGEY V. GAZENKO
1106 Parkside Ln. , Mason , OH 45040
(513) 336-0631 (Home)
(513) 627-5883 (Lab/Work)
(513) 203-0958 (Cell)
E-mail: sgazenko@cinci.rr.com
Research Experience
Adjunct Assistant Professor Miami University, Department of Chemistry and Biochemistry. Research on the MCA Technology and three SBIR phase I research grants.
January 2004 - Present
Analytical Microbiologist Procter & Gamble Company.
Microbiological laboratory at the Millennium Wing Building , St. Bernard, Cincinnati . Position involves the participation in research on new biological active disinfectants and preservatives for development of new products.
July 2003 – Present
Senior Scientist Scientific Device Laboratory Inc., Des Plains , Illinois.
Position involved research on the new methods of coloration of microbiological samples based on fluorescent dyes and mixtures of dyes. Research on detection of Criptococcus neoformans – deadly dangerous fungal infection.
September 1998 – November 1999
Postdoctoral Research Scientist Bioaerosol Research Laboratory, Environmental Health Department, University of Cincinnati , Cincinnati , Ohio .
Grant from the Center for Indoor Air Research.
Research on enzymatic activity of Actinomycetes spores – important air pollutant, by fluorogenic substrates and dynamic of enzymatic activity during spore germination. Participation in the research on aerosolisation of Fungal and Actinomycetes spore and their detection.
July 1996 – September 1998
Manager of the Microbiological Lab Biomedtechnika Company, Moscow , Russia . Research & Development of the new methods of detection and identification of microorganisms based on artificial substrates and fluorescent dyes. Participation in research, testing, validation of the new sampling instruments and methods for detection of microorganisms.
February 1991 – July 1995
Senior Scientist, Group Leader All-Union Research Institute of Biological Instrument Development, Moscow , Russia . Research on the new rapid and sensitive methods for detection, enumeration and identification of microorganisms based on fluorescent dyes, artificial substrates (chromogenic and fluorogenic) and optical instruments: flow cytometer (light scattering and fluorescent), microphoto- and microfluorometer, light and fluorescent microscopes, spectrofluorometers and spectrophotometers.
January 1988 – February 1991
Research Scientist All-Union Research Institute of Biological Instruments Development, Moscow , Russia . Position involved the application and development of rapid and sensitive methods of detection and determination of microorganisms for use with optical instruments.
November 1983 – January 1988
Research Assistant All-Union Research Institute of Biological Instrument Development, Moscow , Russia . Participation in research of Tetrazolium salts – color and fluorescent indicators of Dehydrogenases in live bacterial cells.
October 1979 – November 1983
Business and Industrial Experience
MicroBio Tech Company General Manager of the company. Promotion of the MCA Technology to the microbiological diagnostics market.
May 2003 – Present
Procter & Gamble Company, Cincinnati , OH . Participation in the quality control of the products. Environmental analysis of industrial water source and air pollutions of facilities.
July 2003 – Present
Meridian Bioscience Inc. Microbiological Quality Assurance Laboratory, Microbiologist. Duties included environmental microbiological monitoring (air, water, surfaces, “clean rooms”, equipment) of industrial facilities. Quality control and quality assurance of the ready production and intermediates (microbial detection, enumeration, identification of pollutants and recommendations for treating and decontamination).
April 2001 – July 2003
Biomedtechnika Company. Moscow , Russia . General Manager of the company.
Position included managing of the research & development, design, production, assembling, advertising, marketing and sale of instruments, devices and methods for microbiological industrial, environmental and biotechnological laboratories.
February 1991- July 1995
Companies: Kirishi Biochemical Plant, Siktivkar Vitamin Factory, Moscow Dairy Factory for Children, Pharmaceutical Plant “Akrihin ”. Temporarily work during work at the All-Union Institute of Biological Instrument Development and Biomedtechnika Company. Study of the production processes and implementation of the new rapid method and devices for detection, enumeration and identification of pollutant microorganisms in industrial processes and for environmental monitoring.
1985-1995
Education
Post Doctoral Assistant The fellowship at the Bioaerosol Research Laboratory, Department of the Environmental Health, Cincinnati , Ohio .
Grant from the Center for Indoor Air Research.
September 1996- September 1998
Doctor of Philosophy in Biology (Biotechnology) The Institute of Applied Biochemistry, Moscow , Russia . Dissertation: “Development of the new sensitive express-methods of detection and differentiation of microorganisms for food and biotechnological industry”.
January 1989- April 1991
Master of Science Degree in Biology and Bachelor Degree in Education Moscow State Pedagogical Institute. Department of the Biology and Chemistry. Education has been completed in 4 years instead of 5 years needed regularly. Evaluation of the Diploma done by Globe Language Service, Inc. (319 Broadway, New York , NY 10007 , and fax: 212-693-1489).
September 1975 – July 1979
Bacteriologist Course on medical and environmental bacteriology. All-Union Research Institute for Biological Instrument Development, Moscow , Russia .
January 1980 – July 1980
Course on Professional Computer Use David Computer Information Center , New York , N.Y.
September 1995 – December 1995
Publications
1. S. Gazenko, Method for Detection of Microorganisms by Changing the Shape of Micro Colonies (with MCGP, optical method), US PTO, Reg. #19249, filing date 07/28/2003
2. S. Gazenko, Micromethod and Device for Rapid Detection, Enumeration and Identification of Entities (MCGP, fluorescence, light absorbance), Patent Application, US PTO, #10/141,677, filing date 05/09/2002
3. Gazenko, S., Reponen, T.A., Grinshpun, S.A., and Willeke, K. Analysis of Airborn Actinomycete Spores with Fluorogenic Substrates (Fluorescent method), Applied and Environmental Microbiology, Vol. 64, No. 11, p. 4410-4415, 1998
4. Reponen, T., Gazenko, S., Grinshpun , S.A. , Willeke, K., and Cole, E. Characteristics of Airborne Actinimycete Spores, Applied and Environmental Microbiology , Vol, 64, No. 10, p. 3807-3812, 1998
5. S. Gazenko, T.A. Reponen, S.A. Grinshpun, K. Willeke Determination of Airborne Actinimycete Spores Using Their Enzymatic Activities (Fluorescent method for detection of enzymatic activities) Abstracts of the 98-th General Meeting of the American Society for Microbiology, Atlanta , May 17-21, p. 425, 1998
6. T.A. Reponen, S.V. Gazenko, S.A. Grinshpun, K. Willeke, E.C. Cole Behavior of Airborne Actinimycete Spores in Indoor Air. 6-th International Congress on Aerobiology, Perugia, Italy, August 31 – September 5, p. 86, 1998
7. Willeke, K, Grinshpun, S., Aizenberg, V., Gazenko, S “New bioaerosol sample collection and analysis methods” Proceedings of the NSWC Information Exchange on Bio-Aerosol Sample Collection, Dahlgren, Virginia, March 16-18, p. 107-112, 1998
8. K. Willeke, S. Grinshpun, T. Reponen, T., Lin, S. Gazenko Physical and Biological Aspects of Bioaerosol Generation, Sampling and Analysis International Symposium on Airborne Biological and Abiological Particles, Preila, Neringa, Lithuania, September 10-13, p. 5-13, 1998
9. Gazenko, S. Portable glass inhaler for individual usage, Russian Patent – Certificate, #1803, 1996
10. Gazenko, S., Davidov, Y., Vavilova, L. Method for rapid detection of live microorganisms (Fluorescent method), Patent of Russian Federation #1778189, 1992
11. Gazenko, S. Development of New Rapid and Sensitive Methods for Detection and Differentiation of Microorganisms for Food and Biotechnological Industry, Ph.D. Dissertation, Institute of Applied Biochemistry, Moscow, Russian Federation, 1991
12. Gazenko, S., Koroliov, N. Method for cultivation control of microorganisms (Fluorescent method) Patent of Russian Federation #1564193, 1990
13. Gazenko, S. Method for rapid identification of microorganisms, Patent of Russian Federation #1440917, 1988
14. Gazenko, S., Vorobiov, S., Zemliakov, V., Khookhlaev, K. Method for rapid detection of microorganisms (Double fluorescent coloration of cells), Patent of Russian Federation #1246601, 1986
15. Gazenko, S. Fluorogenic Substrates. Review. All-Union Institute for Biological Instrument Development, Ministry of Medical and Microbiological Industry, Moscow, Russian Federation, 1986
16. Gazenko, S Method for protein detection on indoor surfaces (Sensitive fluorescent detection by Fluorescamine). All-Union Research Institute for Biological Instrument Development, Ministry of Medical and Microbiological Industry, Moscow, Russian Federation, 1983
17. S. Gazenko participated in several other reports and publications on sensitive detection, enumeration and identification of microorganisms mainly based on light absorbance, fluorescence and flow cytometry.
Methods and devices implemented in industry
1. Method for rapid Identification/differentiation of microbes using Formazan – Tetrazolium salts profiles after preliminary growth. Differentiation of four species of Candida genus for Kirishi Biotechnological Factory ( Kirishi , Russia ). This method based on mixture of different Tetrazolium salts and coloration of cells colonies after preliminary growth on solid nutrient media. Method gives opportunity to differentiate Candida maltosa 899 (producent of the fodder protein) from Candida tropicalis, Candida utilis, and Candida rugosa (pollutant microorganisms).
1987
2. Detection of a small amount of surviving live cells in the fodder protein production after sterilization of biomass. Method has been done for Kirishi Biochemical Plant. It is based on mixture of fluorogenic substrates: 4-Methylumbelliferil acetate and 4-Methylumbelliferil butyrate. Method allows sensitive and fast detection of surviving cells: 1000 cells per ml during 1 hour.
1988
3. Vibrotermostat VT-1. Designed for intermixing and thermostatic control of two IFA plates. Instrument has been designed and produced by Biomedtechnika Company and sold to microbiological and immunological laboratories.
1992
4. Reusable Dosing Apparatus for microbiological, biochemical and immunological laboratories. Continuous liquid dosage with adjustable dosage volume. Device has been designed, produced and sold by Biomedtechnika company.
1993
5. Aerosol and bioaerosol samplers PU-ER/220 and PU-ER/12. Instrument allows to sample bioaerosols and aerosols from air on special filters or 5 Petri plates. Sampler has been designed, produced and sold by Biomedtechnika Company.
1994
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