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Biotech / Medical : Ciphergen Biosystems(CIPH):
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To: tuck who wrote (468)10/8/2006 12:26:08 PM
From: tuck  Read Replies (1) of 510
 
[Interest of major serum protein removal for SELDI proteomic blood profiling]

This is a variation of the technique of filtering out some common high abundance proteins via HPLC.

>>Proteome Sci. 2006 Oct 5;4(1):20 [Epub ahead of print]

Interest of major serum protein removal for Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) proteomic blood profiling.

Roche S, Tiers L, Provansal M, Piva MT, Lehmann S.

ABSTRACT: BACKGROUND: Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) has been proposed as new approach for blood biomarker discovery. However, results obtained so far have been often disappointing as this technique still has difficulties to detect low-abundant plasma and serum proteins. RESULTS: We used a serum depletion scheme using chicken antibodies against various abundant proteins to realized a pre-fractionation of serum prior to SELDI-TOF profiling. Depletion of major serum proteins by immunocapture was confirmed by 1D and 2D gel electrophoresis. SELDI-TOF analysis of bound and unbound (depleted) serum fractions revealed that this approach allows the detection of new low abundant protein peaks with satisfactory reproducibility. CONCLUSIONS: The combination of immunocapture and SELDI-TOF analysis opens new avenues into proteomic profiling for the discovery of blood biomarkers.<<

Cheers, Tuck
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