[In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra]
>>J Proteome Res. 2007 Mar 21; [Epub ahead of print]
In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.
Ekblad L, Baldetorp B, Ferno M, Olsson H, Bratt C.
Department of Oncology, Lund University, Se-221 85, Lund, Sweden.
SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.<<
Not sure what these folks mean by "in-source decay," but it sounds as though sample prep and calibration aren't the only issues for getting accurate spectra. Sigh.
Cheers, Tuck |
