CB1 antagonists induce rat chow-seeking behavior..... it's no wonder that humans lost weight in clinical testing!!...... PetFood Express closes at 9 p.m.!!
Am J Physiol Regul Integr Comp Physiol. 2008 Apr 30 [Epub ahead of print]
Cannabinoid CB1 receptor antagonists reduce caloric intake by decreasing palatable diet selection in a novel dessert protocol in female rats.
Mathes CM, Ferrara M, Rowland NE.
Department of Psychology, University of Florida, Gainesville, Florida, United States.
Although many feeding protocols induce obesity, few use multiple foods to analyze diet selection within a single group of animals. To this end, we describe a protocol using time-limited access to a dessert that induces hyperphagia and body weight gain, while allowing simple analysis of diet selection. Female retired breeder Sprague Dawley rats were provided with ad libitum access to standard moist chow (1.67 kcal/g) and daily 8 h nocturnal access to either a sugar gel (SG, 0.31 kcal/g) or sugar fat whip dessert (SFW, 7.35 kcal/g) for 15 days, and food intake and body weight measured daily. Rats given SFW reduced moist chow intake, but not enough to compensate for the large amount of calories consumed from SFW, thus gaining weight. We use this SFW overconsumption protocol to investigate the hypothesis that cannabinoid CB1 receptor antagonists reduce caloric intake by selectively decreasing consumption of palatable foods. In two experiments, female retired breeder Sprague Dawley rats were injected with either Rimonabant (1 mg/kg IP) or vehicle (equal parts polyethylene glycol and saline, 1 ml/kg IP) for 7 days, or one of three doses of AM251 (0.3, 1.0, or 3.0 mg/kg IP) or vehicle for 15 days, and food intake and body weight measured daily. Both Rimonabant and AM251 decreased 24 h caloric intake, but the reduction was specific to a decrease in SFW consumption. This supports the hypothesis that these CB1 receptor antagonists impact feeding by modulating the perception of palatability. |
