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AIL Treatment AIL with purity ³98% was purchased from Shanghai Solarbio Co., Ltd. (Shanghai, P.R. China). AIL was dissolved in complete culture medium of various concentrations, and VS cells were treated for 48 h. Culture medium without AIL served as the control group.
RESULTS
AIL Inhibits VS Cell Proliferation VS cells were treated with different concentrations of AIL (0, 0.2, 0.4, 0.6, 0.8, and 1 µM), and then cell viability was detected. As the results show in Figure 2A, viability of VS cells was reduced by the addition of AIL in a dose-dependent manner; higher concentrations of AIL possessed lower viability. The IC50 value of AIL in VS cells was 0.48 ± 0.023 µM, and 0.6 µM was selected as the AIL concentration used in the following investigations.
Effect of AIL on VS cell proliferation was then detected by BrdU staining. Data displayed in Figure 2B show that BrdU+ cell rate was significantly reduced by the addition of 0.6 µM AIL (p < 0.001). qRT-PCR and Western blot analyses were performed to assess the expression changes of cyclin D1. Results showed that both the mRNA and protein expressions of cyclin D1 were remarkably reduced by AIL administration
More so that the "Tree of Heaven" has some 260 active chemical substances. Ailanthus altissima is a plant of the genus Ailanthus in the family Simaroubaceae. As a traditional Chinese medicine, it has a long history in China; for example, its bark, root bark, and fruit have been used for the treatment of many illnesses. The dried bark from the trunk and root is used for medicine. Until recently, the tree of heaven was used only in folk medicine. But now, it is being investigated as a potential drug. n human phytotherapy the drug prepared from the bark is used orally at doses of 6 to 9 gr. daily
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