Department of Clinical Microbiology, University Hospital, Uppsala,
Sweden.
[Medline record in process]
The aims of this study were to evaluate the sensitivity, specificity,
positive predictive value (PPV), negative predictive value
(NPV) and diagnostic accuracy of a rapid serum and whole-blood test,
Quick Vue One-Step H. pylori test (Quidel
Corporation) for detection of Helicobacter pylori infection. We tested
79 patients with gastrointestinal symptoms, including 8
gastric cancer patients. In total, 116 rapid tests were performed: both
serum and whole-blood samples were tested in 37
patients (74 tests) and serum only was tested in a further 42 patients
(42 tests). Serology by ELISA, bacterial culture and
histology were also carried out on samples from these patients and used
as standards for determining H. pylori infection.
Diagnostic accuracy of the tests was 0.76 for both sera and whole-blood
when culture and histology was used as the standard
in the calculations. However, using serology by ELISA as the standard
raised the diagnostic accuracy to 0.08 in serum samples
and to 0.89 in whole-blood samples. Overall, the H. pylori status
observed when using the rapid tests correlated well with
serology, bacterial culture and histology in this study. |
