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Biotech / Medical : Ligand (LGND) Breakout!
LGND 203.18-1.4%Nov 28 9:30 AM EST
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To: squetch who wrote (15999)2/27/1998 9:09:00 PM
From: Henry Niman  Read Replies (1) of 32384
 
Here's the 4th paper:

Cell 1997 May 2;89(3):365-371

Repression by Ume6 involves recruitment of a complex
containing Sin3 corepressor and Rpd3 histone deacetylase
to target promoters.

Kadosh D, Struhl K

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,
Boston, Massachusetts 02115, USA.

Sin3 and Rpd3 negatively regulate a diverse set of yeast genes. A mouse Sin3-related protein is a
transcriptional corepressor, and a human Rpd3 homolog is a histone deacetylase. Here, we show
that Sin3 and Rpd3 are specifically required for transcriptional repression by Ume6, a DNA-binding
protein that regulates genes involved in meiosis. A short region of Ume6 is sufficient to repress
transcription, and this repression domain mediates a two-hybrid and physical interaction with Sin3.
Coimmunoprecipitation and two-hybrid experiments indicate that Sin3 and Rpd3 are associated in a
complex distinct from TFIID and Pol II holoenzyme. Rpd3 is specifically required for repression by
Sin3, and artificial recruitment of Rpd3 results in repression. These results suggest that repression by
Ume6 involves recruitment of a Sin3-Rpd3 complex and targeted histone deacetylation.

PMID: 9150136, UI: 97294380
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