Panretin (9-cis RA) and small cell lung CA
ncbi.nlm.nih.gov
Biochem Biophys Res Commun 1998 Jan 14;242(2):369-375
Effects of retinoic acid (all-trans and 9-cis) on tumor progression in
small-cell lung carcinoma.
Guzey M, Demirpence E, Criss W, DeLuca HF
TUBITAK-MAM, Research Institute for Genetic Engineering and Biotechnology, Gebze, Turkey.
meral@nucleus.mamgen.tubitak.gov.tr
The two stereoisomers of retinoic acid (RA), all-trans and 9-cis-RA, are regulators of cell proliferation, differentiation and
apoptosis. In this study, the aim was to evaluate the effects of all-trans-and 9-cis-RA on cell growth, proliferation, and on the
induction of apoptosis in the human small cell lung carcinoma (SCLC) cell lines NCI-H82 and NCI-H209. The application of
various concentrations of all-trans and 9-cis-RA were able to inhibit cell growth and proliferation. Moreover, 3H-thymidine
incorporation was inhibited and the number of viable cells decreased, suggesting that all-trans-RA and 9-cis-RA can inhibit cell
proliferation in a dose dependent manner. Morphological examinations (light, electron and fluorescence microscopy)
demonstrated that both retinoids had profound effects on the induction of apoptosis. Our investigation also showed that,
compared to all-trans-RA, 9-cis-RA is a stronger inducer for the inhibition of cell growth and proliferation and that it is more
effective in the induction of apoptosis in small cell lung carcinoma cells in culture.
PMID: 9446801, UI: 98113359 |
