socgenmicrobiol.org.uk
International Journal of Systematic Bacteriology (1998) 48, 511-518
Staphylococcus succinus sp. nov., isolated from Dominican amber
L. H. Lambert,1 T. Cox,2 K. Mitchell,2 R. A. Rossell¢-Mora,3 C. Del Cueto,4 D. E. Dodge,5 P. Orkand,4 R. J. Cano1
1XOMA Corporation, 2910 Seventh Street, Berkeley, CA 94710, USA
2Environmental Biotechnology Institute, California Polytechnic State University, San Luis Obispo, CA 93407, USA
3Instituto Mediterraneo de Estudios Avanzados (CSIC-UIB), Crtra. Valldemosa Km 7.5, Palma de Mallorca, Spain
4Instituto de Neurobiolog¡a, Universidad de Puerto Rico, San Juan, PR 00901, Puerto Rico
5PE Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA
Author for correspondence: L. H.Lambert
Tel: +1 510 644 1170 ext. 2014. Fax: +1 510 841 7805.
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Keywords: Staphylococcus succinus sp. nov., Dominican amber.
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Two bacterial isolates, designated AMG-D1T and AMG-D2, were recovered from 25-35-million-year-old Dominican amber. AMG-D1T and AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically from other staphylococci. Fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. Moreover, AMG-D1T and AMG-D2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the G+C-rich coryneform bacteria, as opposed to L-lysine characteristic of staphylococci. AMG-D1T and AMG-D2 have a G+C ratio of 35 mol%. Phylogenetic analysis with the 16S rRNA gene indicated that AMG-D1T and AMG-D2 were most closely related to Staphylococcus equorum, S. xylosus, Staphylococcus saprophyticus and other novobiocin-resistant staphylococci. Stringent DNA-DNA hybridization studies with AMG-D1T revealed similarities of 38% with S. equorum, 23% with S. xylosus and 6% with S. saprophyticus. The results indicate that AMG-D1T and AMG-D2 represent a novel species, which was named Staphylococcus succinus sp. nov. The type strain of the new species is AMG-D1 (ATCC 700337).
c International Union of Microbiological Societies 1998 |
