From what I can see, the priority date seems to be 7/18/89.....
5776502 : Methods of transcriptionally modulating gene expression
20 CLAIMS
What is claimed is:
1. A method for transcriptionally modulating the expression of a gene of interest in a multicellular organism which
comprises administering to said organism an amount of a molecule effective to modulate transcription of the gene of
interest,
wherein said gene of interest is operably linked to a nucleotide sequence regulating transcription of said gene,
which nucleotide sequence comprises a promoter and 5' nucleotide regulatory sequences of said gene,
wherein said molecule has a molecular weight of 2,000 daltons or leas, does not naturally occur in the organism,
and specifically modulates transcription of the gene of interest by:
1) binding directly to said nucleotide sequence regulating transcription, or
2) by binding directly to RNA produced by transcription from the gene of interest, or
3) by binding directly to a protein regulating transcription of said gene, with the proviso that said molecule
binds the protein at a site distinct from the normal ligand binding site if the protein is a receptor, and
wherein said binding of said molecule results in the transcriptional modulation of the expression of the gene of
interest within said organism.
2. A method of claim 1, wherein the molecule directly binds to said protein.
3. A method of claim 1, wherein the multicellular organism is a human being.
4. A method of claim 1, wherein the multicellular organism is an animal.
5. A method of claim 1, wherein the multicellular organism is a plant.
6. A method of claim 1, wherein the gene of interest is associated with modulation of the expression of the symptoms
of a disorder.
7. A method of claim 6, wherein the disorder is selected from the group consisting of cancer, hematopoetic
dysfunction, diabetes, tissue inflammation, atherosclerosis, viral infections, dysfunctions of memory or learning, and
dysfunctions in a cholesterol or other metabolic pathway.
8. A method of claim 4, wherein the gene of interest is a growth hormone gene and the organism is a cow, a pig, a
bird, a fish, a sheep, or a horse.
9. A method of claim 5, wherein the gene of interest is associated with an agronomically important trait.
10. A method of claim 1, wherein the administering comprises topical contact.
11. A method of claim 1, wherein the administering comprises oral, transdermal, intravenous, intramuscular or
subcutaneous administration.
12. A method of claim 1, wherein the gene of interest encodes a hematopoietic protein.
13. A method of claim 12, wherein the hematopoietic protein is a colony stimulating factor.
14. A method of claim 13, wherein the colony stimulating factor is GM-CSF, G-CSF, M-CSF or EPO.
15. A method of claim 1, wherein the gene of interest encodes an interleukin.
16. A method of claim 15, wherein the interleukin is IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7 or IL-8.
17. A method of claim 1, wherein the gene of interest encodes a growth hormone.
18. A method of claim 17, wherein the growth hormone is human, bovine, porcine, avian, ovine, piscine or equine
growth hormone.
19. A method of claim 1, wherein the gene of interest encodes an oncogene.
20. A method of claim 19, wherein the oncogene is phl-abl, H-ras, N-ras, K-ras, neu, or src. |
