Any views on patent 5851802? Claims follow below:
Claims
1. A recombinant DNA vector construct suitable for introduction into a bacterial host comprising a coding sequence for a
fusion protein having: (a) at least one cationic bactericidal/permeability-increasing (BPI) peptide encoding DNA
sequence; (b) a carrier protein encoding DNA sequence; and (c) an amino acid cleavage site encoding DNA sequence
located between the sequences (a) and (b), wherein the construct additionally encodes a bacterial secretory leader
sequence at the amino-terminus of the fusion protein and wherein the encoded BPI peptide has anti-bacterial activity.
2. The vector construct of claim 1, wherein the coding sequence for the fusion protein is 5'-(b)-(c)-(a)-3'.
3. The vector construct of claim 1, wherein the anti-bacterial encoded BPI peptide also has anti-fungal activity.
4. The vector construct of claim 1, wherein the anti-bacterial encoded BPI peptide also has endotoxin-binding activity.
5. The vector construct of claim 1, wherein the anti-bacterial encoded BPI peptide also has heparin-binding activity.
6. The vector construct of claim 1, wherein the encoded carrier protein is a cationic carrier protein.
7. The vector construct of claim 1, wherein cationic carrier protein is selected from the group of gelonin and the D
subunit of human osteogenic protein.
8. The vector construct of claim 1, wherein the encoded BPI peptide has an amino acid sequence given in any one of the
sequences SEQ ID NOS. 1-239.
9. The vector construct of claim 1, wherein the encoded amino acid cleavage site is selected from the group of codons
encoding Asp-Pro, Met, Trp and Glu.
10. A bacterial host cell transformed with the vector construct of claim 1.
11. An E. coli host cell according to claim 10.
12. A method for bacterial production of a cationic BPI peptide comprising the steps of:
(a) culturing a transformed bacterial host cell according to claim 10 under conditions allowing expression therein of the
fusion protein;
(b) isolating the expressed fusion protein;
(c) cleaving the expressed fusion protein to release the cationic BPI peptide; and
(d) isolating the cationic BPI peptide.
13. A method for bacterial production of a cationic BPI peptide comprising the steps of:
(a) culturing a transformed bacterial host cell according to claim 10 under conditions allowing expression therein of the
fusion protein;
(b) cleaving the expressed fusion protein to release the cationic BPI peptide; and
(c) isolating the cationic BPI peptide.
14. A method for bacterial production of a fusion protein comprising the steps of:
(a) culturing a transformed bacterial host cell according to claim 10 under conditions allowing expression therein of the
fusion protein; and
(b) isolating the expressed fusion protein.
15. The fusion protein product of the process of claim 14. |
