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Biotech / Medical : Essential Therapeutics (ETRX) formerly Microcide (MCDE -- Ignore unavailable to you. Want to Upgrade?

To: sim1 who wrote (282)	8/19/2000 11:09:37 AM
From: scaram(o)uche	Read Replies (1) \| Respond to of 415

In Vitro Activity of Aztreonam, Fosfomycin, Gentamicin or Levofloxacin against Gram-Negative Bacteria in the Presence of the Cephalosporins RWJ-54428 (MC-02,479) or RWJ-333441 (MC-04,546) B. FOLENO, E. WIRA, K. BUSH The R. W. Johnson Pharmaceutical Res. Inst., Raritan, NJ Presentation Number: 1075 Background: RWJ-54428 and RWJ-333441 are cephalosporins with targeted activity against multi-drug resistant gram-positive pathogens, particularly MRSA. If either of these agents were to be used for empiric therapy or in combination therapy for treatment of polymicrobial infections, it is necessary to confirm that the in vitro activity of antibacterial agents with activity against gram-negative bacteria is not altered by the presence of the cephalosporins. Methods: Each cephalosporin was combined with aztreonam, fosfomycin, gentamicin or levofloxacin, and MICs were determined using a checkerboard technique in agar dilution assays. Interaction of these combinations was evaluated against 30 strains of enterobacteriaceae, 4 strains of Pseudomonas aeruginosa and 2 strains of Moraxella catarrhalis. Fractional inhibitory concentrations were determined using the criteria that an FIC £0.5 correlated with synergy and an FIC >4 with antagonism. Results: Although an occasional strain exhibited synergy with each combination, no consistent synergistic pattern was seen for any species with any of the four agents. Indifference was seen for all four combinations in at least 24 of the 36 strains. No antagonism was observed for any combination. Conclusion: On the basis of in vitro results, these two gram-positive cephalosporins could be combined with any of these four gram-negative active agents for successful empiric therapy.

To: sim1 who wrote (282)	8/19/2000 11:34:29 AM
From: John O'Neill	Read Replies (1) \| Respond to of 415

Thanks for the info....perhaps you work in the industry?? I find the articles on use of the efflux pump to enhance /potentiate other tx's most promising

To: sim1 who wrote (282)	8/19/2000 1:20:49 PM
From: tuck	Read Replies (1) \| Respond to of 415

S. SILEN & T. ROCKRAT Silicon Investor Bile-ogical Association (SIBIA), Somewhere in Ca. The effects of chronic capillary development on forearm adenosine triphosphate (ATP) depletion and lactic acid expression in acute murine depression models. In this dose ranging study a subject experienced in murine depression (mouse clicking) was monitored while continuously cutting and pasting abstracts for two hours. We hoped to determine whether or not chronic administration of several hours per day of intermittent murine depression led to sufficient capillary enhancement of the forearm to allow the subject to withstand acute murine depression for shorter periods of time. The subject has been observed intermittently cutting and pasting for years. In this initial study, the dose was two continuous hours, as the subject stopped for breakfast. ATP and lactic acid levels were monitored every 5 minutes, and a laboratory standard MS Mouse was used for the murine depression model. After half an hour ATP depletion reached equilibrium with lactic acid expression and remained stable for the remaing 90 minutes till breakfast, with the subject continuously operating at 90% of his fastest murine depression. Our results indicate that chronic intermittent murine depression can lead to the kind of forearm capillary development that allows acute murine depression. Further study is needed to see how long this acute murine depression lasts. Stuart, are you done with breakfast yet? Back in front of your monitor, you rat! All kidding aside, many thanks for your efforts. I'm finding the directions they're taking in screening and bioarrays particularly interesting. I'm hardly omniscient in this area, but I don't know of another outfit with MCDE's focus here. Cheers, Tuck (aka RockRat)