Silicon Investor (SI) -- The First Internet Community

STOCKTALK

We've detected that you're using an ad content blocking browser plug-in or feature. Ads provide a critical source of revenue to the continued operation of Silicon Investor. We ask that you disable ad blocking while on Silicon Investor in the best interests of our community. If you are not using an ad blocker but are still receiving this message, make sure your browser's tracking protection is set to the 'standard' level.

Biotech / Medical : PROTEOMICS -- Ignore unavailable to you. Want to Upgrade?

To: nigel bates who wrote (94)	8/24/2000 12:38:50 PM
From: nigel bates	Read Replies (1) \| Respond to of 539

And... "Phylos is developing the PROfile™ chip, the first high-throughput addressable array of capture proteins, which will revolutionize drug discovery and functional proteomics. With this chip, scientists will have the power to rapidly assess the protein expression profile of a biological sample so as to measure the relative level of hundreds and ultimately thousands of different proteins simultaneously. Differential analysis of these profiles can then be used to identify the proteins in human tissues that are affected as a result of a particular disease or in response to a specific therapeutic drug. The many applications for the PROfile™ chip include: * Expression profiling, for target identification and validation * Toxicology profiling, for drug lead selection * Diagnostic evaluation, for patient management * Disease management, for therapy selection PROfile™ Chip: An addressable array of capture proteins that can be used to rapidly measure the protein levels in biological samples... Proprietary Technologies Phylos has developed a highly advanced set of proprietary technologies to expedite the development of its PROfile™ chip: * High-throughput Capture Protein Selection PROfusion™, Phylos’ automated protein selection platform, is a state-of-the-art method for obtaining the large numbers of high-affinity capture proteins required by the high-density PROfile™ chip. Phylos is rapidly expanding its capacity to 1,000 selections per month by the end of 2000. Because all of the capture proteins will be selected under similar selection conditions, these arrays can be designed to function optimally under conditions that are compatible with the biological sample being analyzed. Using the automated PROfusion™ platform, new capture proteins can be rapidly selected and added to an array as required. * Stable Capture Proteins Phylos has established a proprietary position with an antibody-like molecule. This structure offers a number of attributes that are critical in the development of a protein chip: * Stability, including a melting point of 110° C * Ease of manufacture * Ultra high-affinity (picomolar) * Selective binding * High packing density * Attachment Chemistries Through the development of the PROfusion™ platform, Phylos scientists have patented several novel attachment schemes for anchoring proteins to solid surfaces. The key features of these attachment schemes are: * Uniform orientation * Density maximization * Detection The PROfile™ chip is particularly amenable to the use of multiple detection systems: * Mass spectrometry * Fluorescence * Surface plasmon resonance Phylos has used each of these methods to detect target proteins on the chip surface, and the Company continues to evaluate various strategies for detecting bound protein targets. Chip Specifications Phylos has validated the PROfile™ chip using peptides as well as its proprietary antibody mimic scaffold as capture molecules. The chip currently utilizes feature sizes of 200 microns in a 2,000-feature array; that is, only the size of a glass coverslip. A highly sensitive working range of more than three orders of magnitude has been readily achieved, and it is anticipated that this range will increase significantly in the future. A Superior Technology Platform The Phylos technology provides a rapid, high-throughput solution for the production of high-density protein arrays. A key strength is the ability to automate the discovery of capture proteins with properties necessary to be chip-compatible. Phylos’ automated combinatorial biology platform provides the best possible solution for this task due to the initial library sizes that are used to select the capture proteins - Phylos’ libraries are at least 10,000 times the size of those of competitors - and the speed at which large varieties of binders can be generated. Protection of Intellectual Property The Phylos PROfile™ chip is a breakthrough product in the emerging field of protein biochips. In order to capitalize on this opportunity, the Company has protected each element involved in its development as proprietary intellectual property that will provide Phylos and its partners with a sustainable competitive advantage. "