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Biotech / Medical : Trickle Portfolio -- Ignore unavailable to you. Want to Upgrade?

To: tuck who wrote (317)	1/1/2001 1:09:29 PM
From: tuck	Respond to of 1784

I think I may have found one of the more important and elusive trickle Industry Associations: abrf.org Wasn't even sure till I looked at the list of corporate sponsors, and found that almost all of them are in the portfolio or watchlist. Pretty plain website. Can't help but notice that their conference is in my hometown in a month and a half. Along with many others in the field over the next few months. Any SI biofreaks planning on attending a San Diego conference or on just being in town are welcome to let me know, and we can hook up for beer, if you like. Happy New Millenium, All. Off soon to enjoy its first day. Cheers, Tuck

To: tuck who wrote (317)	1/2/2001 7:38:49 PM
From: Pseudo Biologist	Read Replies (1) \| Respond to of 1784

Tuck, if I understand this, we are talking of a very fundamental limitation, one that people have known about for many years and unlikely to be "fixed" one of these days. The original article on FRET by a man named Forster was published in 1948; BRET, of course, is a slight twist on the basic FRET concept. The fundamental limitation is in the physics of (F/B)RET whereby donor/acceptor partners need to be within some critical distance for the measurement to be possible. For particular proteins one could, I guess, tweak the system to make it work. This would not be possible in general protein-protein mapping proteomics projects, but may be doable for small molecule screening and a given pair of interacting proteins. If you really, really want to use the BRET system and your pair does not appear to interact by BRET even though you know it does, then tweaking at the molecular biology level may allow you to "fix" the problem for the particular case. An interesting survey may be found in ncbi.nlm.nih.gov (admittedly full-text would be a lot more informative here, but I think you get a flavor from the abstract on the main issues at hand: this is where I would start with the "tweaking") Turns out a very tricklish, formerly very active, SI contributor, Richard Haugland of Molecular Probes, would know this area hands down. If anyone is willing to "resurrect" him ... PB