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To: tnsaf who wrote (1464)	2/3/2002 5:29:57 PM
From: tnsaf	Respond to of 7143

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae Brian J. Akerley, Eric J. Rubin, Veronica L. Novick, Kensey Amaya, Nicholas Judson, and John J. Mekalanos,§ * Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115; and Department of Microbiology Molecular Genetics, Harvard Medical School, Boston, MA 02115 Proc. Natl. Acad. Sci. USA, Vol. 99, Issue 2, 966-971, January 22, 2002 A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function. ------------------------------------------------------------ www.pnas.org/cgi/doi/10.1073/pnas.012602299