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Biotech / Medical : 3-DIMENSIONAL PHARMACEUTICALS (DDDP) -- Ignore unavailable to you. Want to Upgrade?

To: scaram(o)uche who wrote (77)	6/28/2002 6:03:06 PM
From: keokalani'nui	Read Replies (2) \| Respond to of 146

>>The integrin adhesion receptors for vitronectin and osteopontin, avb3 and avb5, are essential modulators of angiogenesis and endothelial cell adhesion and have been implicated in tumor angiogenesis, atherosclerosis and restenosis<< and..... Virology 2002 May 25;297(1):31-8 Books, LinkOut Involvement of Integrin alphavbeta3 in the Pathogenesis of Human Immunodeficiency Virus Type 1 Infection in Monocytes. Lafrenie RM, Lee SF, Hewlett IK, Yamada KM, Dhawan S. Northeastern Ontario Regional Cancer Centre, Sudbury, Ontario, P3E 5J1, Canada Attachment of HIV to macrophages is a critical early event in the establishment of infection. In the present study, we demonstrate the involvement of integrin alphavbeta3 (vitronectin receptor) in HIV infection of peripheral blood monocyte-derived macrophages. Culturing monocytes in the presence of M-CSF for 3 days upregulated expression of the alphav-containing integrins, alphavbeta3 and alphavbeta5. The increase in alphavbeta3 expression was accompanied by increased HIV-1 replication by monocytes. Immunoblot analysis showed that purified HIV-gp120 protein interacted with CD4 and alphavbeta3 in immunoprecipitation experiments. Neutralizing antibodies against the alphavbeta3 integrin interfered with the coprecipitation of alphavbeta3 with an anti-gp120 antibody and substantially inhibited HIV infection of monocytes. Neutralizing antibodies against alphavbeta5 or beta1 integrins did not significantly affect HIV infection. These results indicate that HIV infection of primary monocytes requires differentiation of these cells and may involve alphavbeta3 interaction with the HIV-1 envelope protein gp120 for productive infection. (c) 2002 Elsevier Science (USA).