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To: scaram(o)uche who wrote (1301)	12/15/2002 1:43:48 PM
From: scaram(o)uche	Read Replies (1) \| Respond to of 1784

When completed manually, Mullis' PCR technique was slow and labor-intensive. Therefore, Cetus scientists began looking for ways in which to automate the process. Before the discovery of the thermostable Taq enzyme, scientists needed to add fresh enzyme to each cycle. The first thermocycling machine, "Mr. Cycle" was developed by Cetus engineers to address that need to add fresh enzyme to each test tube after the heating and cooling process. And the purification of the Taq polymerase resulted in the need for a machine to cycle more rapidly among different temperatures. In 1985, Cetus formed a joint venture with the Perkin-Elmer Corporation in Norwalk, Connecticut, and introduced the DNA Thermal Cycler. si.edu