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Biotech / Medical : The thread of life -- Ignore unavailable to you. Want to Upgrade?

To: Mike McFarland who wrote (479)	6/1/2003 6:42:07 PM
From: Mike McFarland	Read Replies (2) \| Respond to of 1336

Surfing cell signaling today and found quite a scientific advisory board at Akceli. akceli.com Brugge there (used to be with Ariad) Reverse transfection has several advantages over traditional transfection. A reverse transfection microarray contains a much higher density of genes than the sets of microwells generally used for transfection. A single slide can replace 100 plates containing 96 microwells apiece. That reduces the amount of expensive reagents required for experiments and greatly increases the number of targets that a laboratory can examine - from 100,000 to one million per day. Since the assay is conducted in a single vessel, the method also provides a more uniform environment for reactions than sets of single transfections carried out in microwells, whose contents can vary slightly in condition from one to the next. In addition, reverse transfection is particularly well suited to studying proteins that exist in cell membranes, which are difficult to investigate using traditional techniques. akceli.com ..................................... Just parking the url for a paper on TRAF signaling biochem.wisc.edu --Interesting how complicated this is--I was reading about the IKK complex today. (A lecture by Jay Levy was on the UW cable channel--talking about innate immunity and HIV, somehow that got me over to NFkB, then over to IkappaB).