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Biotech / Medical : XOMA. Bull or Bear? -- Ignore unavailable to you. Want to Upgrade?

To: Robert K. who wrote (16684)	2/28/2004 8:57:13 PM
From: Robert K.	Read Replies (1) \| Respond to of 17367

The ongoing research IS TRUELY AMAZING. Whats even MORE amazing, is that this is the case IN SPITE OF the supposed trial "failures". Biochem Biophys Res Commun. 2003 Nov 28;311(4):853-63. Related Articles, Links AML-1, PU.1, and Sp3 regulate expression of human bactericidal/permeability-increasing protein. Lennartsson A, Pieters K, Ullmark T, Vidovic K, Gullberg U. Department of Hematology, Lund University, Lund, Sweden. Bactericidal/permeability-increasing protein (BPI) is an antimicrobial protein in neutrophils, stored in azurophil granules. Expression of BPI is absent in neutrophils of newborns and patients with secondary granule deficiency (SGD), possibly contributing to dysfunction of neutrophils. We report two alternative transcription start sites at 52 and 22bp upstream of the translation start. A proximal 222bp promoter conferring expression in myeloid cells was identified, and critical cis-acting sites for myeloid expression were contained within the 159bp upstream of translation start. Within this region, direct binding and transactivation by AML-1, PU.1, and Sp3 were demonstrated, as judged by electrophoretic mobility shift analysis. Moreover, transient transfections of C/EBPalpha or C/EBPepsilon to HeLa cells resulted in increased promoter activity, indicating a direct or indirect role for C/EBP. In conclusion, we provide evidence for AML-1, PU.1, and Sp3 cooperatively and directly mediating BPI-expression during myeloid differentiation.