[Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance]
Published online before print May 23, 2005
Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0409920102
OPEN ACCESS ARTICLE
Microbiology
Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance
( genotyping | group A streptococci | infectious disease | Streptococcus pyogenes | pneumonia )
David J. Ecker *, Rangarajan Sampath *, Lawrence B. Blyn *, Mark W. Eshoo *, Cristina Ivy *, Joseph A. Ecker *, Brian Libby *, Vivek Samant *, Kristin A. Sannes-Lowery *, Rachael E. Melton *, Kevin Russell , Nikki Freed , Chris Barrozo , Jianguo Wu , Karl Rudnick , Anjali Desai , Emily Moradi , Duane J. Knize , David W. Robbins , James C. Hannis *, Patina M. Harrell *, Christian Massire *, Thomas A. Hall *, Yun Jiang *, Raymond Ranken *, Jared J. Drader *, Neill White *, John A. McNeil *, Stanley T. Crooke *, and Steven A. Hofstadler *
*Ibis Division of Isis Pharmaceuticals, 1891 Rutherford Road, Carlsbad, CA 92008; Naval Health Research Center, P.O. Box 85122, San Diego, CA 92186-5122; and SAIC, 10260 Campus Point Drive, San Diego, CA 92121
Edited by Stanley Falkow, Stanford University, Stanford, CA, and approved April 11, 2005 (received for review December 31, 2004)
Epidemic respiratory infections are responsible for extensive morbidity and mortality within both military and civilian populations. We describe a high-throughput method to simultaneously identify and genotype species of bacteria from complex mixtures in respiratory samples. The process uses electrospray ionization mass spectrometry and base composition analysis of PCR amplification products from highly conserved genomic regions to identify and determine the relative quantity of pathogenic bacteria present in the sample. High-resolution genotyping of specific species is achieved by using additional primers targeted to highly variable regions of specific bacterial genomes. This method was used to examine samples taken from military recruits during respiratory disease outbreaks and for follow up surveillance at several military training facilities. Analysis of respiratory samples revealed high concentrations of pathogenic respiratory species, including Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pyogenes. When S. pyogenes was identified in samples from the epidemic site, the identical genotype was found in almost all recruits. This analysis method will provide information fundamental to understanding the polymicrobial nature of explosive epidemics of respiratory disease.<<
pnas.org
Full text freebie. This is TIGER, isn't it? I don't think Isis has announced this publication yet (unless they said something on the CC). If not, they will, meaning a potential trading opportunity.
Cheers, Tuck |
